Slc9a8tm1d(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Slc9a8tm1d(KOMP)Wtsi |
Name: |
solute carrier family 9 (sodium/hydrogen exchanger), member 8; targeted mutation 1d, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5925354 |
Synonyms: |
Nhe8- |
Gene: |
Slc9a8 Location: Chr2:167263632-167318920 bp, + strand Genetic Position: Chr2, 87.22 cM
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Alliance: |
Slc9a8tm1d(KOMP)Wtsi page
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IMPC: |
Slc9a8 gene page |
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Mutant Cell Line: |
EPD0033_5_A07 |
Germline Transmission: |
Earliest citation of germline transmission:
J:245977
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Parent Cell Line: |
JM8.F6 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
Vector: L1L2_gt1
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Mutation details: The L1L2_gt1 cassette was inserted at position 167282591 of Chromosome 2 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 167283331. Exon 4 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 4. Western blot analysis confirmed the absence of protein expression in the male and female reproductive system and kidney of adult homozygous mutant mice.
(J:245977)
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Key: |
hm |
homozygous |
ht |
heterozygous |
tg |
involves transgenes |
√ |
phenotype observed |
cn |
conditional genotype |
cx |
complex: > 1 genome feature |
ot |
other: hemizygous, indeterminate,... |
N |
normal phenotype |
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Genotype/ Background: |
| Allelic Composition | Genetic Background | Cell Line(s) |
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Loading... | | | involves: C57BL/6N | EPD0033_5_A07 | | | involves: C57BL/6 * C57BL/6N * DBA/2 | EPD0033_5_A07 |
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Phenotypes: |
Affected Systems |
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cellular
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√
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√
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abnormal sperm mitochondrial sheath morphology
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√
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√
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absent sperm mitochondrial sheath
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√
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√
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abnormal acrosome assembly
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√
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abnormal proacrosomal vesicle fusion
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√
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globozoospermia
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√
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absent acrosome
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√
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√
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abnormal sperm nucleus morphology
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√
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asthenozoospermia
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√
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reproductive system
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√
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√
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reproductive system phenotype
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N
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abnormal sperm mitochondrial sheath morphology
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√
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√
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absent sperm mitochondrial sheath
|
√
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√
|
abnormal acrosome assembly
|
√
|
|
abnormal proacrosomal vesicle fusion
|
√
|
|
globozoospermia
|
√
|
|
absent acrosome
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√
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√
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abnormal sperm nucleus morphology
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√
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asthenozoospermia
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√
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male infertility
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√
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Slc9a8 Mutation: |
108 strains or lines available
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Original: |
J:245977 Oberheide K, et al., Loss of the Na+/H+ exchanger NHE8 causes male infertility in mice by disrupting acrosome formation. J Biol Chem. 2017 Jun 30;292(26):10845-10854 |
All: |
2 reference(s) |
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