Gas2l3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gas2l3^{tm1c(EUCOMM)Hmgu}
• Name: growth arrest-specific 2 like 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5925364
• Synonyms: Gas2l3^fl
• Gene: Gas2l3 Location: Chr10:89244685-89279829 bp, - strand Genetic Position: Chr10, 44.98 cM
• Alliance: Gas2l3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Gas2l3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244087
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 89264650 of Chromosome 10 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 89265497. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:244087)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gas2l3 Mutation: 72 strains or lines available

References

• Original: J:244087 Stopp S, et al., Deletion of Gas2l3 in mice leads to specific defects in cardiomyocyte cytokinesis during development. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):8029-8034
• All: 1 reference(s)