Gas2l3^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gas2l3^{tm1d(EUCOMM)Hmgu}
• Name: growth arrest-specific 2 like 3; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5925365
• Gene: Gas2l3 Location: Chr10:89244685-89279829 bp, - strand Genetic Position: Chr10, 44.98 cM
• Alliance: Gas2l3^{tm1d(EUCOMM)Hmgu} page
• IMPC: Gas2l3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244087
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 89264650 of Chromosome 10 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 89265497. Exon 6 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:244087)

Expression

In Mice Carrying this Mutation:	31 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gas2l3 Mutation: 72 strains or lines available

References

• Original: J:244087 Stopp S, et al., Deletion of Gas2l3 in mice leads to specific defects in cardiomyocyte cytokinesis during development. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):8029-8034
• All: 1 reference(s)