Dach1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dach1^{tm1c(KOMP)Wtsi}
• Name: dachshund family transcription factor 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925368
• Gene: Dach1 Location: Chr14:98024289-98407201 bp, - strand Genetic Position: Chr14, 48.25 cM, cytoband E3
• Alliance: Dach1^{tm1c(KOMP)Wtsi} page
• IMPC: Dach1 gene page

Mutation origin

• Mutant Cell Line: EPD0714_4_C11
• Germline Transmission: Earliest citation of germline transmission: J:243789
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98256190 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 98257419. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:243789)

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dach1 Mutation: 51 strains or lines available

References

• Original: J:243789 Chang AH, et al., DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12-CXCR4 signaling axis. Genes Dev. 2017 Aug 04;31(13):1308-1324
• All: 2 reference(s)