Spata2^em1Jliu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spata2^em1Jliu
• Name: spermatogenesis associated 2; endonuclease-mediated mutation 1, Jiali Liu
• MGI ID: MGI:6093706
• Gene: Spata2 Location: Chr2:167323053-167334804 bp, - strand Genetic Position: Chr2, 87.23 cM
• Alliance: Spata2^em1Jliu page

Mutation origin

• Strain of Origin: ICR

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology targeting exon 2 results in an 1009 bp deletion from the 83 bp after the initiation codon to the 199th base of exon 3. Western blot analysis confirmed the absence of protein expression in testis. (J:247929)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spata2 Mutation: 20 strains or lines available

References

• Original: J:247929 Zhao J, et al., Deletion of Spata2 by CRISPR/Cas9n causes increased inhibin alpha expression and attenuated fertility in male mice. Biol Reprod. 2017 Sep 01;97(3):497-513
• All: 1 reference(s)