Phf8^tm1.1Hoch
Targeted Allele Detail

Summary

• Symbol: Phf8^tm1.1Hoch
• Name: PHD finger protein 8; targeted mutation 1.1, Konrad Hochedlinger
• MGI ID: MGI:6094243
• Gene: Phf8 Location: ChrX:150303668-150416855 bp, + strand Genetic Position: ChrX, 68.46 cM
• Alliance: Phf8^tm1.1Hoch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:248400
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 6 and a neomycin resistance gene cassette and a second loxP site into intron 8. Exons 7 and 8 (and the neo cassette) were subsequently deleted through cre-mediated recombination, resulting in a null allele. Western blots confirmed the loss of protein expression from this allele and RNA-seq confirmed the lack of exon 7 and 8 reads. (J:248400)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Phf8 Mutation: 16 strains or lines available

References

• Original: J:248400 Walsh RM, et al., Phf8 loss confers resistance to depression-like and anxiety-like behaviors in mice. Nat Commun. 2017 May 09;8:15142
• All: 1 reference(s)