Gt(ROSA)26Sor^{tm1(CAG-GFP,-Mars*L274G)Esm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-GFP,-Mars*L274G)Esm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Erin M Schuman
• MGI ID: MGI:6095164
• Synonyms: R26-MetRS*, STOP^FLOXR26-MetRS*
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-GFP,-Mars*L274G)Esm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:248509
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-GFP,-Mars*L274G)Esm} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mars1 (MGI:1345633)	mutated mouse gene resulting in L274G substitution

• Mutation details: The exchange vector is designed to contain a mutant F3 FRT site, a NEO selection cassette, CAG promoter, a floxed STOP cassette, Green Fluorescent Protein coding sequence, foot-and-mouth disease virus 2A peptide sequence (to mediate ribosomal skipping), the mouse methionine-tRNA synthetase (Mars) with the L274G point mutation, and an FRT site. This vector was integrated into the Gt(ROSA)26Sor locus by recombinase mediated cassette exchange (RMCE). The L274G mutation allows the binding of the methionine surrogate azidonorleucine (ANL) onto the methionine tRNA. ANL can be tagged for imaging or affinity purification. (J:248509)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

Notes

Embryonic stem cells = A-A02

References

• Original: J:248509 Alvarez-Castelao B, et al., Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nat Biotechnol. 2017 Dec;35(12):1196-1201
• All: 9 reference(s)