Rorc^tm1.1Zsun
Targeted Allele Detail

Summary

• Symbol: Rorc^tm1.1Zsun
• Name: RAR-related orphan receptor gamma; targeted mutation 1.1, Zuoming Sun
• MGI ID: MGI:6095621
• Synonyms: RORgammat^M
• Gene: Rorc Location: Chr3:94280106-94305583 bp, + strand Genetic Position: Chr3, 40.56 cM, cytoband F2
• Alliance: Rorc^tm1.1Zsun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:248565
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector contains an FRT-flanked neomycin (neo) resistance cassette inserted downstream of exon 3. Exon 4 is modified to harbor alanine substitutions at amino acids 92 and 93 (S92A-L93A). These mutations are located in the hinge domain and are associated with the differentiation of TH17 cells. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:248565)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rorc Mutation: 44 strains or lines available

References

• Original: J:248565 He Z, et al., A two-amino-acid substitution in the transcription factor RORgammat disrupts its function in TH17 differentiation but not in thymocyte development. Nat Immunol. 2017 Oct;18(10):1128-1138
• All: 3 reference(s)