Gpc6^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gpc6^{tm2c(EUCOMM)Wtsi}
• Name: glypican 6; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6098731
• Gene: Gpc6 Location: Chr14:117162727-118213956 bp, + strand Genetic Position: Chr14, 60.34 cM, cytoband E4
• Alliance: Gpc6^{tm2c(EUCOMM)Wtsi} page
• IMPC: Gpc6 gene page

Mutation origin

• Mutant Cell Line: EPD0547_5_C04
• Germline Transmission: Earliest citation of germline transmission: J:245694
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 117672643 of Chromosome 14 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 117673696. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:245694)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpc6 Mutation: 46 strains or lines available

References

• Original: J:245694 Capurro M, et al., Glypican-6 promotes the growth of developing long bones by stimulating Hedgehog signaling. J Cell Biol. 2017 Sep 04;216(9):2911-2926
• All: 1 reference(s)