Tg(C9orf72*)19Lagi
Transgene Detail

Summary

• Symbol: Tg(C9orf72*)19Lagi
• Name: transgene insertion 19, Clotilde Lagier-Tourenne
• MGI ID: MGI:6100942
• Synonyms: C9¹⁹
• Transgene: Tg(C9orf72*)19Lagi Location: unknown
• Alliance: Tg(C9orf72*)19Lagi page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(C9orf72*)19Lagi expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  C9orf72 (203228)

  C9orf72

  (MGI:1920455)

  BAC subclone contains a contraction to 90 or 450 GGGGCC repeats

• Mutation details: The transgenic construct is constructed with a ~156 kbp human bacterial artificial chromosome (BAC) CH523-111K12 (Chr9:27,561,110-27,714,308; May 2016) containing the human C9ALS patient-derived C9orf72 gene encoding a truncated short C9ORF72 isoform (222 aa; exons 1-5 with TAA site and polyA signal) with hexanucleotide repeat expansions (GGGGCC) in intron 1a. The BAC has ~140 kbp of transcriptionally-upstream (telomeric) flanking sequences that contain no other confirmed genes. Founder line C9<19> (called line 19) was identified with multiple transgene copies at the same locus that segregate together - with each transgene copy containing either ~90 or ~450 hexanucleotide repeat lengths. (J:232242)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:232242 Jiang J, et al., Gain of Toxicity from ALS/FTD-Linked Repeat Expansions in C9ORF72 Is Alleviated by Antisense Oligonucleotides Targeting GGGGCC-Containing RNAs. Neuron. 2016 May 4;90(3):535-50
• All: 2 reference(s)