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Variant origin |
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Variant description |
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Notes |
Mapping and Phenotype information for this QTL, its variants and associated markersJ:248161The C57BL/6J (B6) strain is a well-known mouse model of late-onset age related hearing loss and develops severe hearing loss with respect to stimuli at mid frequencies (1-10 kHz) at 9-15 months of age, stimuli at high frequencies (10-20 kHz) at 4-10 months of age, and stimuli at ultra-sonic frequencies (20 kHz) at 2-6 months of age. Contrastingly, the DBA/2J (D2) strain shows early-onset progressive hearing loss (PHL). The hearing loss of D2 mice rapidly progresses to the low frequencies from the ultra-sonic frequencies and to profound levels at approximately 8 months of age.In the current study hearing abilities were measured in B6, D2, F1 and N2 mice using an auditory brain stem response (ABR) workstation to test the mice for ABR thresholds. The thresholds from both ears in all mice were measured using a tone tip stimulus at 8, 16 and 32 kHz. Data for the ABR thresholds from the F1 and N2 populations suggested that the QTL responsible for hearing loss in D2 mice differed according to sound frequency.((DBA/2Jcl x C57BL/6Jcl) F1 x DBA/2Jcl) backcrossed progeny were used to map the susceptibility loci in D2 mice linked to early onset age related hearing loss. DNA samples from 90 N2 mice were genotyped using 103 polymorphic microsatellite markers located throughout the mouse genome, based on size variation between the PCR products from D2 and B6 mice. Linkage mapping was performed by QTL interval mapping using the R/qtl program of the R Statistical package to perform single-QTL genome scans by Haley-Knott regression using a step size of 1.0 cM. The logarithm of odds (LOD) scores for significance were calculated by 1,000 permutations testing using.QTL linkage analysis revealed a QTL on Chromosome 11 with significant LOD scores of 5.01 at 8kHz and 8.84 at 16kHz. The authors state this locus (D11Mit103) was in the same position as the previously mapped QTL Ahl8, indicating a strong effect of the homozygous Fscn2 |
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References |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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