H2-Ab1^g7-em1Dvs
Endonuclease-mediated Allele Detail

Summary

• Symbol: H2-Ab1^g7-em1Dvs
• Name: histocompatibility 2, class II antigen A, beta 1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6101479
• Synonyms: H2-Ab1^em1Dvs
• Gene: H2-Ab1 Location: Chr17:34482201-34488392 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Ab1^g7-em1Dvs page

Mutation origin

• Strain of Origin: NOD/ShiLtDvs-H2-K1^d-em1Dvs H2-D1^b-em5Dvs/Dvs

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cas9 RNA and four guide sequences targeting Exon 2 (CCAACGGGACGCAGCGCATA, CGAAGCGCAGGTACTCCTCC, CGACGTGGGCGAGTACCGCG, ACACAACTACGAGGAGACGG) were injected into NOD/ShiLtDvs-H2-K1^em1Dvs H2-D1^em5Dvs/Dvs embryos yielding this 181bp deletion (ATACGGCTCGTGACCAGATACATCTACAACCGGGAGGAGTACCTGCGCTTCGACAGCGACGTGGGCGAGTACCGCGCGGT GACCGAGCTGGGGCGGCACTCAGCCGAGTACTACAATAAGCAGTACCTGGAGCGAACGCGGGCCGAGCTGGACACGGCGT GCAGACACAACTACGAGGAGA) within Exon 2 of H2-Ab1. Flow cytometry showed a paucity of CD8+ and CD4+ T-cells in the blood, and flow cytometry showing loss of H2-D, H2-K, and I-A on blood B-cells. (J:257229)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2-Ab1 Mutation: 83 strains or lines available

References

• Original: J:257229 Racine JJ, et al., Improved Murine MHC-Deficient HLA Transgenic NOD Mouse Models for Type 1 Diabetes Therapy Development. Diabetes. 2018 May;67(5):923-935
• All: 4 reference(s)