Zc3h14^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zc3h14^{tm1c(KOMP)Wtsi}
• Name: zinc finger CCCH type containing 14; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6107560
• Synonyms: Zc3h14^F
• Gene: Zc3h14 Location: Chr12:98713223-98754012 bp, + strand Genetic Position: Chr12, 49.88 cM, cytoband F1
• Alliance: Zc3h14^{tm1c(KOMP)Wtsi} page
• IMPC: Zc3h14 gene page

Mutation origin

• Mutant Cell Line: EPD0366_5_F01
• Germline Transmission: Earliest citation of germline transmission: J:249492
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98749419 of Chromosome 12 upstream of exon 13 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 13 at position 98750249. Exon 13 is thus flanked by loxP sites. This "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:249492)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zc3h14 Mutation: 50 strains or lines available

References

• Original: J:249492 Rha J, et al., The RNA-binding protein, ZC3H14, is required for proper poly(A) tail length control, expression of synaptic proteins, and brain function in mice. Hum Mol Genet. 2017 Oct 01;26(19):3663-3681
• All: 1 reference(s)