Zc3h14^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zc3h14^{tm1d(KOMP)Wtsi}
• Name: zinc finger CCCH type containing 14; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6107561
• Synonyms: Zc3h14^deltaex13
• Gene: Zc3h14 Location: Chr12:98713223-98754012 bp, + strand Genetic Position: Chr12, 49.88 cM, cytoband F1
• Alliance: Zc3h14^{tm1d(KOMP)Wtsi} page
• IMPC: Zc3h14 gene page

Mutation origin

• Mutant Cell Line: EPD0366_5_F01
• Germline Transmission: Earliest citation of germline transmission: J:249492
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98749419 of Chromosome 12 upstream of exon 13 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 13 at position 98750249. Exon 13 is thus flanked by loxP sites. The lacZ-neo cassette was removed by in vitro flp recombinase expression. Subsequent cre expression cre-mediated recombination removed exon 13, creating a null allele. (J:249492)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zc3h14 Mutation: 50 strains or lines available

References

• Original: J:249492 Rha J, et al., The RNA-binding protein, ZC3H14, is required for proper poly(A) tail length control, expression of synaptic proteins, and brain function in mice. Hum Mol Genet. 2017 Oct 01;26(19):3663-3681
• All: 3 reference(s)