Hltf^tm1.1Hdin
Targeted Allele Detail

Summary

• Symbol: Hltf^tm1.1Hdin
• Name: helicase-like transcription factor; targeted mutation 1.1, Hao Ding
• MGI ID: MGI:6108112
• Gene: Hltf Location: Chr3:20111975-20172654 bp, + strand Genetic Position: Chr3, 6.15 cM
• Alliance: Hltf^tm1.1Hdin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252991
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The genomic sequence for the coding part of exon 1 plus exons 2-5 (coding for amino-acids 2-208) was replaced with a lacZ reporter gene cassette and a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent Cre-mediated recombination, leaving a combined reporter null allele. Northern blot and RT-PCR analysis confirmed the absence of endogenous transcripts from this allele. (J:252991)

Expression

In Mice Carrying this Mutation:

43 assay results

Tumor Data

List all tumor models in MMHCdb carrying Hltf^tm1.1Hdin

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hltf Mutation: 47 strains or lines available

References

• Original: J:252991 Sandhu S, et al., Loss of HLTF function promotes intestinal carcinogenesis. Mol Cancer. 2012 Mar 27;11:18
• All: 2 reference(s)