Hyal3^tm1Btr
Targeted Allele Detail

Summary

• Symbol: Hyal3^tm1Btr
• Name: hyaluronoglucosaminidase 3; targeted mutation 1, Barbara Triggs-Raine
• MGI ID: MGI:6108181
• Gene: Hyal3 Location: Chr9:107458495-107464558 bp, + strand Genetic Position: Chr9, 58.19 cM
• Alliance: Hyal3^tm1Btr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144606
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette (neo) was inserted into exon 2 of the gene at the NsiI site. This targeting construct was designed to disrupt Hyal3 while having minimal impact on an overlapping gene, Nat6 (N-acetyltransferase 6), which second exon is embedded within the first intron of this gene. RT-PCR analysis showed that no wild type transcript is expressed from this allele, and that Fus2 trasncripts are not altered in testis, but the levels are reduced about 20% in liver. (J:144606)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hyal3 Mutation: 11 strains or lines available

References

• Original: J:144606 Atmuri V, et al., Hyaluronidase 3 (HYAL3) knockout mice do not display evidence of hyaluronan accumulation. Matrix Biol. 2008 Oct;27(8):653-60
• All: 2 reference(s)