Kpna7^tm1.1Sgao
Targeted Allele Detail

Summary

• Symbol: Kpna7^tm1.1Sgao
• Name: karyopherin subunit alpha 7; targeted mutation 1.1, Shaorong Gao
• MGI ID: MGI:6108355
• Synonyms: Kpna7^mut
• Gene: Kpna7 Location: Chr5:144920537-144946446 bp, - strand Genetic Position: Chr5, 84.91 cM
• Alliance: Kpna7^tm1.1Sgao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244973
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5 and 6 were replaced with a floxed Pgk-neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette. RT-PCR analysis confirmed the absence of the short, predominant, 1500 bp transcript but detected the presence of a second 1215 bp transcript that skips exons 5 and 6 and contains a novel exon 7. However, western blot analysis did not detect either protein product. (J:244973)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kpna7 Mutation: 21 strains or lines available

References

• Original: J:244973 Hu J, et al., Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse. J Biol Chem. 2010 Oct 22;285(43):33113-22
• All: 1 reference(s)