App^tm1.2Tlyp
Targeted Allele Detail

Summary

• Symbol: App^tm1.2Tlyp
• Name: amyloid beta precursor protein; targeted mutation 1.2, Tracy L Young-Pearse
• MGI ID: MGI:6111357
• Synonyms: APP gKO
• Gene: App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
• Alliance: App^tm1.2Tlyp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241006
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 2 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 3. Flp-mediated recombination removed the FRT-flanked neo cassette. Exon 3 was removed through cre-mediated recombination. Western blot analysis demonstrates the absence of protein. (J:241006)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any App Mutation: 105 strains or lines available

References

• Original: J:241006 Callahan DG, et al., Embryonic mosaic deletion of APP results in displaced Reelin-expressing cells in the cerebral cortex. Dev Biol. 2017 Apr 15;424(2):138-146
• All: 1 reference(s)