Gk5^em1Btlr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gk5^em1Btlr
• Name: glycerol kinase 5; endonuclease-mediated mutation 1, Bruce Beutler
• MGI ID: MGI:6111536
• Synonyms: Gk5^D280N
• Gene: Gk5 Location: Chr9:96001415-96066661 bp, + strand Genetic Position: Chr9, 50.31 cM
• Alliance: Gk5^em1Btlr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Single point mutation
• Mutation details: A G>A point mutation was introduced using the CRISPR/Cas9 system and sgRNA 5'-CTCCAAACATGGCCGACTGC-3'. The point mutation in the kinase domain changes aspartic acid codon 280 to an asparagine codon (p.Asp280Asn or p.D280N) and creates a kinase-dead allele. Glycerol kinase activity assays showed kinase activity reduced to less than 10% of wild-type. (J:243852)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gk5 Mutation: 44 strains or lines available

References

• Original: J:243852 Zhang D, et al., Skin-specific regulation of SREBP processing and lipid biosynthesis by glycerol kinase 5. Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206
• All: 1 reference(s)