Gk2^em1Btlr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gk2^em1Btlr
• Name: glycerol kinase 2; endonuclease-mediated mutation 1, Bruce Beutler
• MGI ID: MGI:6111538
• Synonyms: Gk2^- CRISPR 1
• Gene: Gk2 Location: Chr5:97603001-97604880 bp, - strand Genetic Position: Chr5, 47.71 cM
• Alliance: Gk2^em1Btlr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 257 bp deletion was created using the CRISPR/Cas9 system and sgRNA 5'-CCAGATGAGCCAAGAGTCGA-3'. This deletion in the 3' part of the coding region causes a reading frameshift. (J:243852)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gk2 Mutation: 20 strains or lines available

References

• Original: J:243852 Zhang D, et al., Skin-specific regulation of SREBP processing and lipid biosynthesis by glycerol kinase 5. Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206
• All: 1 reference(s)