Axin2^em1Fstl
Endonuclease-mediated Allele Detail

Summary

• Symbol: Axin2^em1Fstl
• Name: axin 2; endonuclease-mediated mutation 1, Frank Staal
• MGI ID: MGI:6111808
• Synonyms: Axin2-TQ
• Gene: Axin2 Location: Chr11:108811175-108841609 bp, + strand Genetic Position: Chr11, 71.83 cM
• Alliance: Axin2^em1Fstl page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/J)F1 x C57BL/6JIcoCrlLumc

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 mediated mutatgenesis was used to replace most of exon 2 (including the start codon) with an mTurquoise2 (green fluorescent protein in far blue) coding sequence and a loxP-flanked neomycin resistance (neo) cassette. Reporter expression is under the control of endogenous gene promoter. (J:252680)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Axin2 Mutation: 44 strains or lines available

References

• Original: J:252680 de Roo JJD, et al., Axin2-mTurquoise2: A novel reporter mouse model for the detection of canonical Wnt signalling. Genesis. 2017 Oct;55(10)
• All: 2 reference(s)