Med28^tm1.2Vra
Targeted Allele Detail

Summary

• Symbol: Med28^tm1.2Vra
• Name: mediator complex subunit 28; targeted mutation 1.2, Vijaya Ramesh
• MGI ID: MGI:6111946
• Gene: Med28 Location: Chr5:45677571-45686618 bp, + strand Genetic Position: Chr5, 25.02 cM
• Alliance: Med28^tm1.2Vra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243694
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two loxP sites flanking exons 1 and 2 and a neomycin resistance cassette in reverse orientation flanked by FRT sites within intron 2 were inserted via homologous recombination. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exons 1 and 2. (J:243694)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med28 Mutation: 16 strains or lines available

References

• Original: J:243694 Li L, et al., Mediator Subunit Med28 Is Essential for Mouse Peri-Implantation Development and Pluripotency. PLoS One. 2015;10(10):e0140192
• All: 1 reference(s)