Sccpdh^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sccpdh^em1(IMPC)J
• Name: saccharopine dehydrogenase (putative); endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6112025
• Gene: Sccpdh Location: Chr1:179495796-179514754 bp, + strand Genetic Position: Chr1, 83.62 cM
• Alliance: Sccpdh^em1(IMPC)J page
• IMPC: Sccpdh gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CTATAGGCAGGTGATCTAGG, TATCTGACCCAATTATAGAG, ACATTAGAGAGGGGGACACA and GTTTGTTCTGATACAAAGAA, which resulted in a 576 bp deletion beginning at Chromosome 1 positive strand position 179,670,350 bp and ending after 179,670,925 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001069513 (exon 2) and 463 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 63 and early truncation 13 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sccpdh Mutation: 19 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)