Miga2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Miga2^{tm1c(KOMP)Wtsi}
• Name: mitoguardin 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6112036
• Synonyms: Miga2^flox
• Gene: Miga2 Location: Chr2:30254245-30275533 bp, + strand Genetic Position: Chr2, 21.63 cM
• Alliance: Miga2^{tm1c(KOMP)Wtsi} page
• IMPC: Miga2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234466
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 30258415 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30259370. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:234466)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Miga2 Mutation: 36 strains or lines available

References

• Original: J:234466 Liu XM, et al., Mitoguardin-1 and -2 promote maturation and the developmental potential of mouse oocytes by maintaining mitochondrial dynamics and functions. Oncotarget. 2016 Jan 12;7(2):1155-67
• All: 3 reference(s)