Tcra^Ln1Sdou
Allele Detail

Summary

• Symbol: Tcra^Ln1Sdou
• Name: T cell receptor alpha chain; recombination line 1, Stephanie Dougan
• MGI ID: MGI:6113295
• Gene: Tcra Location: Chr14:52665424-54461655 bp, + strand Genetic Position: Chr14, cytoband D1-D2

Mutation origin

• Strain of Origin: (C57BL/6 x 129)F1

Mutation description

• Allele Type: Other (Not Specified)
• Mutation: Other
• Mutation details: This allele is generated using somatic cell nuclear transfer (SCNT). Individual (C57BL/6 x 129)F1 donor nuclei from iNKT (invariant natural killer T) cells were isolated and used to replace the mitotic spindle from mouse oocytes. Cells are developed to the blastocyst stage and used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. The iNKT cells were selected to carry a rearranged invariant Tcra V14-J18 segment. (J:254145)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tcra Mutation: 97 strains or lines available

References

• Original: J:254145 Clancy-Thompson E, et al., Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets. J Immunol. 2017 Jul 1;199(1):159-171
• All: 2 reference(s)