Hes7tm2.1Ybes
Targeted Allele Detail
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| Symbol: |
Hes7tm2.1Ybes |
| Name: |
hes family bHLH transcription factor 7; targeted mutation 2.1, Yasumasa Bessho |
| MGI ID: |
MGI:6113393 |
| Synonyms: |
Hes710k |
| Gene: |
Hes7 Location: Chr11:69011230-69014881 bp, + strand Genetic Position: Chr11, 42.38 cM
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| Alliance: |
Hes7tm2.1Ybes page
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A lox71 site, a neomycin resistance gene cassette and a lox2272 site were inserted into the 3' UTR in exon 4 immediately after the stop codon. Successfully targeted ES cells were transfected with a cre-expressing vector and a vector containing part of intron 74 of the human dystrophin (DMD) gene. This vector was constructed as follows: a lox66 site, genomic sequence from the last 11 bp of exon 74 up to the first 10 bp of exon 75, and a lox2272 site. The intronic sequence was shortened to 10 kb by deleting sequence from the central portion. Cre-mediated recombination recombined the genomic lox71 site with the vector lox66 site and the genomic and vector lox2272 sites with each other. This results in a knock-in allele with part of the human intron and parts of its flanking exons inserted into the 3' UTR intron of the host gene. RT-PCR, 3'-RACE and in situ hybridization experiments showed that the exogenous intron is not spliced out.
(J:244271)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hes7 Mutation: |
22 strains or lines available
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| Original: |
J:244271 Fujimuro T, et al., Hes7 3'UTR is required for somite segmentation function. Sci Rep. 2014 Sep 24;4:6462 |
| All: |
1 reference(s) |
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