Hes7^tm2.1Ybes
Targeted Allele Detail

Summary

• Symbol: Hes7^tm2.1Ybes
• Name: hes family bHLH transcription factor 7; targeted mutation 2.1, Yasumasa Bessho
• MGI ID: MGI:6113393
• Synonyms: Hes7^10k
• Gene: Hes7 Location: Chr11:69011230-69014881 bp, + strand Genetic Position: Chr11, 42.38 cM
• Alliance: Hes7^tm2.1Ybes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244271
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lox71 site, a neomycin resistance gene cassette and a lox2272 site were inserted into the 3' UTR in exon 4 immediately after the stop codon. Successfully targeted ES cells were transfected with a cre-expressing vector and a vector containing part of intron 74 of the human dystrophin (DMD) gene. This vector was constructed as follows: a lox66 site, genomic sequence from the last 11 bp of exon 74 up to the first 10 bp of exon 75, and a lox2272 site. The intronic sequence was shortened to 10 kb by deleting sequence from the central portion. Cre-mediated recombination recombined the genomic lox71 site with the vector lox66 site and the genomic and vector lox2272 sites with each other. This results in a knock-in allele with part of the human intron and parts of its flanking exons inserted into the 3' UTR intron of the host gene. RT-PCR, 3'-RACE and in situ hybridization experiments showed that the exogenous intron is not spliced out. (J:244271)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hes7 Mutation: 23 strains or lines available

References

• Original: J:244271 Fujimuro T, et al., Hes7 3'UTR is required for somite segmentation function. Sci Rep. 2014 Sep 24;4:6462
• All: 1 reference(s)