Tcrb^Ln2Sdou
Allele Detail

Summary

• Symbol: Tcrb^Ln2Sdou
• Name: T cell receptor beta chain; recombination line 2, Stephanie Dougan
• MGI ID: MGI:6113625
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Strain of Origin: (C57BL/6 x 129)F1

Mutation description

• Allele Type: Other (Not Specified)
• Mutation: Other
• Mutation details: This allele is generated using somatic cell nuclear transfer (SCNT). Individual (C57BL/6 x 129)F1 donor nuclei from iNKT (invariant natural killer T) cells were isolated and used to replace the mitotic spindle from mouse oocytes. Cells are developed to the blastocyst stage and used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. The Tcrb allele selected for propagation from the transferred nuclei had a productive, in-frame recombination of the Vbeta7 gene segments with a unique CDR3 C sequence (CASSLPGHNERLFF). (J:254145)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 95 strains or lines available

References

• Original: J:254145 Clancy-Thompson E, et al., Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets. J Immunol. 2017 Jul 1;199(1):159-171
• All: 1 reference(s)