TcrbLn3Sdou
Allele Detail
|
Symbol: |
TcrbLn3Sdou |
Name: |
T cell receptor beta chain; recombination line 3, Stephanie Dougan |
MGI ID: |
MGI:6113677 |
Gene: |
Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C
|
|
|
Allele Type: |
|
Other (Not Specified) |
Mutation: |
|
Other
|
|
|
Mutation details: This allele is generated using somatic cell nuclear transfer (SCNT). Individual (C57BL/6 x 129)F1 donor nuclei from iNKT (invariant natural killer T) cells were isolated and used to replace the mitotic spindle from mouse oocytes. Cells are developed to the blastocyst stage and used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. The Tcrb allele selected for propagation from the transferred nuclei had a productive, in-frame recombination of the Vbeta 8.2 gene segment and a unique CDR3 sequence (CASSDRGYEQYF).
(J:254145)
|
|
|
|
Original: |
J:254145 Clancy-Thompson E, et al., Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets. J Immunol. 2017 Jul 1;199(1):159-171 |
All: |
1 reference(s) |
|