Cdkn2a^em1Cjs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdkn2a^em1Cjs
• Name: cyclin dependent kinase inhibitor 2A; endonuclease-mediated mutation 1, Charles J Sherr
• MGI ID: MGI:6114043
• Synonyms: p15^smArf
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^em1Cjs page

Mutation origin

• Strain of Origin: C57BL/6J x FVB/N

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: RNAs encoding forward and reverse TALEN nucleases directed to sequences flanking the first Arf ATG translation initiation codon were injected into C57BL/6J FVB/N mouse zygotes. This resulted in one strain with a 25 bp deletion deleting the ATG and another with a 1 bp deletion immediately downstream of the ATG. Either of these deletions forces the use of the ATG downstream at codon position 45, yielding the shorter p15^smArf peptide. The 25 bp deletion strain was used for further analysis. (J:244260)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 67 strains or lines available

References

• Original: J:244260 van Oosterwijk JG, et al., Small mitochondrial Arf (smArf) protein corrects p53-independent developmental defects of Arf tumor suppressor-deficient mice. Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):7420-7425
• All: 1 reference(s)