Tdrd5^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tdrd5^{tm1c(EUCOMM)Hmgu}
• Name: tudor domain containing 5; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6115347
• Synonyms: Tdrd5^fl
• Gene: Tdrd5 Location: Chr1:156082866-156131234 bp, - strand Genetic Position: Chr1, 67.71 cM, cytoband G3
• Alliance: Tdrd5^{tm1c(EUCOMM)Hmgu} page
• IMPC: Tdrd5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255733
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 156115550 of Chromosome 1 upstream of exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon at position 156116441. Exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 7. (J:255733)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tdrd5 Mutation: 41 strains or lines available

References

• Original: J:255733 Ding D, et al., TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 2018 Jan 9;9(1):127
• All: 1 reference(s)