Sec22b^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec22b^{tm1c(EUCOMM)Wtsi}
• Name: SEC22 homolog B, vesicle trafficking protein; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6115369
• Synonyms: Sec22b^fl
• Gene: Sec22b Location: Chr3:97808543-97829634 bp, + strand Genetic Position: Chr3, 42.35 cM
• Alliance: Sec22b^{tm1c(EUCOMM)Wtsi} page
• IMPC: Sec22b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255769
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 97819459 of Chromosome 3 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 97820325. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 3. (J:255769)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec22b Mutation: 15 strains or lines available

References

• Original: J:255769 Wu SJ, et al., A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-Presentation. Cell Rep. 2017 Jun 27;19(13):2645-2656
• All: 2 reference(s)