Plp1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Plp1^{tm1c(EUCOMM)Wtsi}
• Name: proteolipid protein (myelin) 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6115637
• Synonyms: Plp^flox
• Gene: Plp1 Location: ChrX:135723420-135740482 bp, + strand Genetic Position: ChrX, 59.1 cM, cytoband F1-F2
• Alliance: Plp1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Plp1 gene page

Mutation origin

• Mutant Cell Line: EPD0160_2_D10
• Germline Transmission: Earliest citation of germline transmission: J:245100
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 135732265 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 135733444. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:245100)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plp1 Mutation: 18 strains or lines available

References

• Original: J:245100 Luders KA, et al., Genetic dissection of oligodendroglial and neuronal Plp1 function in a novel mouse model of spastic paraplegia type 2. Glia. 2017 Nov;65(11):1762-1776
• All: 5 reference(s)