Myo10^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Myo10^{tm1d(KOMP)Wtsi}
• Name: myosin X; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6115841
• Synonyms: Myo10^tm1d
• Gene: Myo10 Location: Chr15:25622636-25813759 bp, + strand Genetic Position: Chr15, 9.36 cM, cytoband C
• Alliance: Myo10^{tm1d(KOMP)Wtsi} page
• IMPC: Myo10 gene page

Mutation origin

• Mutant Cell Line: EPD0332_3_B02
• Germline Transmission: Earliest citation of germline transmission: J:256438
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25785401 of Chromosome 15 upstream of the critical exon (exon 27) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 27 at position 25786400. Exon 27 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 27. Immunoblots of whole brain from P5 pups confirmed that expression of both the full-length and headless forms is undetectable in homozygous mutant mice. (J:256438)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myo10 Mutation: 612 strains or lines available

References

• Original: J:256438 Heimsath EG Jr, et al., Myosin-X knockout is semi-lethal and demonstrates that myosin-X functions in neural tube closure, pigmentation, hyaloid vasculature regression, and filopodia formation. Sci Rep. 2017 Dec 11;7(1):17354
• All: 3 reference(s)