Ppm1l^tm1Tkoba
Targeted Allele Detail

Summary

• Symbol: Ppm1l^tm1Tkoba
• Name: protein phosphatase 1 (formerly 2C)-like; targeted mutation 1, Takayasu Kobayashi
• MGI ID: MGI:6117305
• Synonyms: ppm1l^delta
• Gene: Ppm1l Location: Chr3:69224251-69468131 bp, + strand Genetic Position: Chr3, 32.21 cM
• Alliance: Ppm1l^tm1Tkoba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238086
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The beta-galactosidase (lacZ) neomycin gene cassette, consisting of a lox71 site, lacZ, frt site, neomycin gene, frt site, and loxP site, was inserted into the 5'UTR sequence of exon 1 via homologous recombination, replacing a portion of exon 1 which contains the translational initiation site. Western blot analysis confirmed absence of protein in brain and liver. (J:238086)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ppm1l Mutation: 26 strains or lines available

References

• Original: J:238086 Kusano R, et al., Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain. FEBS Lett. 2016 Oct;590(20):3606-3615
• All: 1 reference(s)