Tg(Cacng5-cre)181Tjmc
Transgene Detail

Summary

• Symbol: Tg(Cacng5-cre)181Tjmc
• Name: transgene insertion 181, Thomas J McHugh
• MGI ID: MGI:6117425
• Synonyms: Cacng5-Cre
• Transgene: Tg(Cacng5-cre)181Tjmc Location: unknown
• Alliance: Tg(Cacng5-cre)181Tjmc page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Cacng5-cre)181Tjmc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cacng5 (MGI:2157946)

• Mutation details: The bacterial artificial chromosome (BAC) RP23-145G23 containing mouse calcium channel, voltagedependent, gamma subunit 5 (Cacng5) gene was modified to insert a Cre-FRT-Amp-FRT cassette at the start site of Cacng5 translation. The ampicillin (Amp) marker was deleted and the Cre-modified BAC's were confirmed, purified and injected into C57BL/6 fertilized pronuclei. Five founders (lines 160, 181, 190, 212, 225) were generated. The 181 Cacng5-Cre line was determined to express Cre selectively in the CA2 area of hippocampus using immunohistochemistry with the anti-Cre antibody, and used for analyses. (J:256034)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cacng5 (mouse) Summary of all recombinase alleles driven by Cacng5. Comparative matrix view of recombinase activities Alliance mouse Cacng5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:256034 Boehringer R, et al., Chronic Loss of CA2 Transmission Leads to Hippocampal Hyperexcitability. Neuron. 2017 May 3;94(3):642-655.e9
• All: 4 reference(s)