Mir137^tm1.2Mtm
Targeted Allele Detail

Summary

• Symbol: Mir137^tm1.2Mtm
• Name: microRNA 137; targeted mutation 1.2, Michael McManus
• MGI ID: MGI:6118102
• Gene: Mir137 Location: Chr3:118227506-118227578 bp, + strand Genetic Position: Chr3, 51.71 cM
• Alliance: Mir137^tm1.2Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244465
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and a third loxP site immediately downstream of the microRNA stem loop. The reporter and selection cassettes were deleted through flp-mediated recombination. This was intended to create a functional conditional-ready allele but the parent allele Mir137^tm1Mtm, which was intended to be a reporter knockout allele, was shown to lack beta-galactosidase expression, suggesting that the inserted FRT and/or LoxP recombination sites interfere with the transcription of the allele. Consequently, this allele remains a null allele and did not turn into a conditional-ready allele. (J:244465)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir137 Mutation: 5 strains or lines available

References

• Original: J:244465 Crowley JJ, et al., Disruption of the microRNA 137 primary transcript results in early embryonic lethality in mice. Biol Psychiatry. 2015 Jan 15;77(2):e5-7
• All: 1 reference(s)