Dbh^{em1.1(flpo)Rray}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dbh^{em1.1(flpo)Rray}
• Name: dopamine beta hydroxylase; endonuclease-mediated mutation 1.1, Russell Ray
• MGI ID: MGI:6118955
• Synonyms: DBH-FLPo, Dbh^{tm1(DBH-FLPo)Rray}
• Gene: Dbh Location: Chr2:27055519-27073216 bp, + strand Genetic Position: Chr2, 19.29 cM
• Alliance: Dbh^{em1.1(flpo)Rray} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251840
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Dbh^{em1.1(flpo)Rray} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dbh (MGI:94864)

• Mutation details: The mouse line was generated by homologous recombination using the CRISPR/Cas9 technology. The coding sequences for optimized FLP recombinase was inserted at the transcriptional start site of the endogenous Dbh gene replacing 106 bps of the first exon; a single lox2722 (modified LoxP) site marks the former position of a floxed neomycin resistance cassette that was deleted by Cre-mediated recombination. The resulting allele is a knock-out of Dbh protein product but express Flpo recombinase under control of the Dbh gene. (J:251840)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dbh (mouse) Summary of all recombinase alleles driven by Dbh. Alliance mouse Dbh gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dbh Mutation: 17 strains or lines available

References

• Original: J:251840 Sun JJ, et al., Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016;11(7):e0159474
• All: 1 reference(s)