Gfra1^{tm1.1(cre/ERT2)Cfi}
Targeted Allele Detail

Summary

• Symbol: Gfra1^{tm1.1(cre/ERT2)Cfi}
• Name: glial cell line derived neurotrophic factor family receptor alpha 1; targeted mutation 1, Carlos F Ibanez
• MGI ID: MGI:6119532
• Synonyms: Gfra1^CreERT2
• Gene: Gfra1 Location: Chr19:58224036-58444341 bp, - strand Genetic Position: Chr19, 54.18 cM, cytoband D2-D3
• Alliance: Gfra1^{tm1.1(cre/ERT2)Cfi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251552
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gfra1^{tm1.1(cre/ERT2)Cfi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gfra1 (MGI:1100842)

• Mutation details: The targeting vector using bacterial artificial chromosomes (BACs) from the C57BL/6J RPCIB-731 BAC library was constructed by inserting a CreERT2 cassette and F3 flanked puromycin resistant cassette into the second exon of the Gfra1 gene in frame. The vector was transfected into the TaconicArtemis C57BL/6N Tac ESC line to establish mouse line by homologous recombination. Flp-mediated recombination removed the F3-flanked puro cassette. (J:251552)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gfra1 (mouse) Summary of all recombinase alleles driven by Gfra1. Alliance mouse Gfra1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gfra1 Mutation: 33 strains or lines available

References

• Original: J:251552 Sergaki MC, et al., GFRalpha1 Regulates Purkinje Cell Migration by Counteracting NCAM Function. Cell Rep. 2017 Jan 10;18(2):367-379
• All: 3 reference(s)