Islr^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Islr^{tm1b(EUCOMM)Wtsi}
• Name: immunoglobulin superfamily containing leucine-rich repeat; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6119548
• Synonyms: Meflin^-
• Gene: Islr Location: Chr9:58063547-58066563 bp, - strand Genetic Position: Chr9, 31.63 cM
• Alliance: Islr^{tm1b(EUCOMM)Wtsi} page
• IMPC: Islr gene page

Mutation origin

• Mutant Cell Lines: EPD0788_2_B12, EPD0788_2_H09
• Germline Transmission: Earliest citation of germline transmission: J:244530
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 58063944 of Chromosome 9 upstream of exon 2, between the two alternative exons 1 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene cassette under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 58066013. Exon 2, which contains the entire coding region of the gene, was deleted, together with the neo cassette, through subsequent cre-mediated recombination. (J:244530)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Islr Mutation: 24 strains or lines available

References

• Original: J:244530 Maeda K, et al., Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells. Sci Rep. 2016 Feb 29;6:22288
• All: 4 reference(s)