Zfand2b^tm1Otin
Targeted Allele Detail

Summary

• Symbol: Zfand2b^tm1Otin
• Name: zinc finger, AN1 type domain 2B; targeted mutation 1, Carlos Lopez-Otin
• MGI ID: MGI:6119758
• Synonyms: Zfand2b^-
• Gene: Zfand2b Location: Chr1:75145290-75148270 bp, + strand Genetic Position: Chr1, 38.61 cM, cytoband C3
• Alliance: Zfand2b^tm1Otin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233169
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: A FRT-flanked trapping element consisting of a splice acceptor sequence followed by a neomycin resistance gene and a loxP site were inserted into introns 1-2. A second loxP site was inserted into introns 8-9 so that exons 2-8 were floxed. qRT-PCR analysis demonstrated the loss of mRNA expression in liver, muscle and bone marrow tissue from homozygous mutant mice. Western blot analysis confirmed absence of encoded protein. (J:233169)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfand2b Mutation: 11 strains or lines available

References

• Original: J:233169 Osorio FG, et al., Loss of the proteostasis factor AIRAPL causes myeloid transformation by deregulating IGF-1 signaling. Nat Med. 2016 Jan;22(1):91-6
• All: 1 reference(s)