Cabp2^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Cabp2^{tm1b(KOMP)Mbp}
• Name: calcium binding protein 2; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:6120904
• Gene: Cabp2 Location: Chr19:4131578-4137340 bp, + strand Genetic Position: Chr19, 3.79 cM, cytoband A
• Alliance: Cabp2^{tm1b(KOMP)Mbp} page
• IMPC: Cabp2 gene page

Mutation origin

• Mutant Cell Line: DEPD0003_7_F06
• Germline Transmission: Earliest citation of germline transmission: J:255028
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4134472 of Chromosome 19 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 4135876. Exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exons. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:255028)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cabp2 Mutation: 22 strains or lines available

References

• Original: J:255028 Picher MM, et al., Ca(2+)-binding protein 2 inhibits Ca(2+)-channel inactivation in mouse inner hair cells. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):E1717-E1726
• All: 1 reference(s)