Strip1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Strip1^{tm1b(KOMP)Wtsi}
• Name: striatin interacting protein 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6121053
• Gene: Strip1 Location: Chr3:107519848-107539010 bp, - strand Genetic Position: Chr3, 46.83 cM
• Alliance: Strip1^{tm1b(KOMP)Wtsi} page
• IMPC: Strip1 gene page

Mutation origin

• Mutant Cell Line: EPD0316_1_G03
• Germline Transmission: Earliest citation of germline transmission: J:251816
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 107531479 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 107532505. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:251816)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Strip1 Mutation: 42 strains or lines available

References

• Original: J:251816 Bazzi H, et al., STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E10928-E10936
• All: 1 reference(s)