Spata18^tm1.2Hifa
Targeted Allele Detail

Summary

• Symbol: Spata18^tm1.2Hifa
• Name: spermatogenesis associated 18; targeted mutation 1.2, Hirofumi Arakawa
• MGI ID: MGI:6121375
• Synonyms: Mieap^-
• Gene: Spata18 Location: Chr5:73808722-73836855 bp, + strand Genetic Position: Chr5, 39.03 cM
• Alliance: Spata18^tm1.2Hifa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254117
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 5 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 7. Subsequent flp-mediated recombination removed the neo cassette. Exons 6 and 7 were deleted through subsequent cre-mediated recombination. These two exons code for part of the coiled-coil domains. RT-PCR and western blots on testes samples showed the absence of expression from this allele. (J:254117)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spata18 Mutation: 30 strains or lines available

References

• Original: J:254117 Tsuneki M, et al., Mieap suppresses murine intestinal tumor via its mitochondrial quality control. Sci Rep. 2015 Jul 28;5:12472
• All: 3 reference(s)