Col1a1^{tm2(CMV/tetO-Kdm2b*)Atz}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(CMV/tetO-Kdm2b*)Atz}
• Name: collagen, type I, alpha 1; targeted mutation 2, Alexandros Tzatsos
• MGI ID: MGI:6121399
• Synonyms: Tet-Kdm2b^deltaJmjc
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(CMV/tetO-Kdm2b*)Atz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232437
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm2(CMV/tetO-Kdm2b*)Atz} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Kdm2b	Expresses a demethylation deficient form

• Mutation details: The construct contains cDNA of demethylation deficient Kdm2b under the control of the CMV promoter fused with the tet-responsive element (tetO) which was co-electroporated into KH2 embryonic stem cells along with a plasmid expressing Flpe recombinase to allow FRT/Flpase-mediated single copy site-specific integration into the locus. (J:232437)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:232437 Andricovich J, et al., Histone demethylase KDM2B regulates lineage commitment in normal and malignant hematopoiesis. J Clin Invest. 2016 Mar 1;126(3):905-20
• All: 1 reference(s)