Gtsf2^tm1.1Miya
Targeted Allele Detail

Summary

• Symbol: Gtsf2^tm1.1Miya
• Name: gametocyte specific factor 2; targeted mutation 1.1, Jun-ichi Miyazaki
• MGI ID: MGI:6121439
• Gene: Gtsf2 Location: Chr15:103347384-103356896 bp, - strand Genetic Position: Chr15, 58.96 cM
• Alliance: Gtsf2^tm1.1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251285
• Parent Cell Line: KY1.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and FRT-flanked pgk-puro cassette were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 4 via homologous recombination. Cre-mediated recombination removed the pgk-puro cassette and exons 2-4. RT-PCR and western blot analyses confirmed the absence of expression in testes from homozygous mice at 8 weeks of age. (J:251285)

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gtsf2 Mutation: 13 strains or lines available

References

• Original: J:251285 Takemoto N, et al., Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis. PLoS One. 2016;11(3):e0150390
• All: 1 reference(s)