Iftap^tm1Mnz
Targeted Allele Detail

Summary

• Symbol: Iftap^tm1Mnz
• Name: intraflagellar transport associated protein; targeted mutation 1, Michel C Nussenzweig
• MGI ID: MGI:6140227
• Synonyms: Nwc^tmpro1
• Gene: Iftap Location: Chr2:101391124-101459376 bp, - strand Genetic Position: Chr2, 53.83 cM, cytoband E2
• Alliance: Iftap^tm1Mnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254587
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s), No functional change)
• Mutation: Intragenic deletion
• Mutation details: A self-excising cassette, containing a tACE promoter, a cre gene, HSV-TK poly(A) sequences and a neomycin resistance gene cassette, was used to target the promoter. This resulted in a 644 bp deletion encompassing the promoter and exon 1. The deleted sequence is also part of intron 2 of the Rag2 gene. RT-PCR experiments show that Rag2 is not affected, but that the targeted locus shows transcription from an alternative promoter with an alternative first exon 1b. (J:254587)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Iftap Mutation: 21 strains or lines available

References

• Original: J:254587 Laszkiewicz A, et al., Complexity of transcriptional regulation within the Rag locus: identification of a second Nwc promoter region within the Rag2 intron. Immunogenetics. 2011 Mar;63(3):183-7
• All: 2 reference(s)