Col15a1^tm2.1Pih
Targeted Allele Detail

Summary

• Symbol: Col15a1^tm2.1Pih
• Name: collagen, type XV, alpha 1; targeted mutation 2.1, Taina Pihlajaniemi
• MGI ID: MGI:6147361
• Synonyms: loxP flanked allele
• Gene: Col15a1 Location: Chr4:47208161-47313167 bp, + strand Genetic Position: Chr4, 26.01 cM, cytoband B1-B3
• Alliance: Col15a1^tm2.1Pih page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was introduced ~900 bp upstream of exon 1 and a loxP-flanked neomycin resistance cassette 120 bp downstream of exon 2. Cre recombinase deleted the selection cassette, leaving exons 1 and 2, encoding the first 33 amino acids of the protein, flanked by loxP sites. This allele was derived from the same targeting event as Col15a1^tm1Pih. (J:67212)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col15a1 Mutation: 71 strains or lines available

References

• Original: J:67212 Eklund L, et al., Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice. Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1194-9
• All: 2 reference(s)