Slc5a5^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc5a5^{tm1d(KOMP)Wtsi}
• Name: solute carrier family 5 (sodium iodide symporter), member 5; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6147653
• Synonyms: NIS KO
• Gene: Slc5a5 Location: Chr8:71335533-71345401 bp, - strand Genetic Position: Chr8, 34.36 cM, cytoband C1
• Alliance: Slc5a5^{tm1d(KOMP)Wtsi} page
• IMPC: Slc5a5 gene page

Mutation origin

• Mutant Cell Line: EPD0860_2_D04
• Germline Transmission: Earliest citation of germline transmission: J:250121
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 71341753 of Chromosome 8 upstream of the critical exons 6 and 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6 and 7 at position 71342751. The critical exons 6 and 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Cre-mediated recombination removed the critical exons 6 and 7. (J:250121)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc5a5 Mutation: 38 strains or lines available

References

• Original: J:250121 Ferrandino G, et al., An extremely high dietary iodide supply forestalls severe hypothyroidism in Na+/I- symporter (NIS) knockout mice. Sci Rep. 2017 Jul 13;7(1):5329
• All: 3 reference(s)