Spata16^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spata16^em1Osb
• Name: spermatogenesis associated 16; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka U
• MGI ID: MGI:6147689
• Synonyms: Spata16^em1Maik, Spata16^{em1(R284Q)Osb)}, Spata16^pm
• Gene: Spata16 Location: Chr3:26691769-27037361 bp, + strand Genetic Position: Chr3, 10.74 cM, cytoband A3
• Alliance: Spata16^em1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258202
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Single point mutation
• Mutation details: A point mutation (G to A) was introduced in codon 851 of exon 4, predicted to result in a R284Q alteration in the encoded protein. This mouse mutation corresponds to the single amino acid substitution (c.848G>A or R283Q) identified in infertile globozoospermic patients. RT-PCR analysis indicated that the point mutation caused no splicing abnormality. (J:258202)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spata16 Mutation: 37 strains or lines available

Notes

EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb.

References

• Original: J:258202 Fujihara Y, et al., Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Int J Mol Sci. 2017 Oct 21;18(10):2208
• All: 1 reference(s)