Spata16^em2Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spata16^em2Osb
• Name: spermatogenesis associated 16; endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka U
• MGI ID: MGI:6147690
• Synonyms: Spata16^-781
• Gene: Spata16 Location: Chr3:26691769-27037361 bp, + strand Genetic Position: Chr3, 10.74 cM, cytoband A3
• Alliance: Spata16^em2Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258202
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR mutagenesis was used to generate a 781 bp deletion that removed 88 bp out of 90 bp of exon 4 which encodes the C-terminus of the tetratricopeptide repeat (TPR) domain. This deletion resulted in the mistranslation of the remaining 2 bp of the mutant fourth exon. (J:258202)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Spata16 Mutation: 37 strains or lines available

Notes

EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb.

References

• Original: J:258202 Fujihara Y, et al., Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Int J Mol Sci. 2017 Oct 21;18(10):2208
• All: 1 reference(s)